| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Escherichia fergusonii | TGCTCTGCAATGCCGTGA | AACGGCAATGCGGATGGA | 59.97 | 60.05 | 165 | 82 |
100.00%
|
50.00%
|
| Primer 82 | Escherichia fergusonii | ACGGCACATTCGCAGTCA | CAGAAAGCAGGCTGTCCCA | 59.97 | 59.93 | 273 | 82 |
100.00%
|
50.00%
|
| Primer 83 | Escherichia fergusonii | CGCGTCGAACCTGTTGTTG | TCTCGTGCATTGCGCTCA | 60.08 | 60.05 | 179 | 82 |
100.00%
|
50.00%
|
| Primer 84 | Escherichia fergusonii | TTGTGGGTGCCAGGTTGA | TTCGCTGCCCTTCACTGT | 58.99 | 59.17 | 188 | 82 |
100.00%
|
50.00%
|
| Primer 85 | Escherichia fergusonii | TTTTGGTGCGTTCGCTGC | CCGCTACGTGCTTCAGTGA | 59.97 | 60.08 | 177 | 82 |
100.00%
|
50.00%
|
| Primer 86 | Escherichia fergusonii | TTGCTGCCAGCCAGATCA | TTTTCTGTGCGCACTGCC | 59.24 | 59.28 | 199 | 82 |
100.00%
|
50.00%
|
| Primer 87 | Escherichia fergusonii | TGCGACGTATTGTGCGCA | TGACGACGTAACCACTGGC | 60.74 | 60.01 | 276 | 81 |
100.00%
|
50.00%
|
| Primer 88 | Escherichia fergusonii | ACCAGTTCAGCAGCTGCA | GGCGACTTCCTTTTGTGCG | 59.49 | 60.08 | 192 | 81 |
100.00%
|
50.00%
|
| Primer 89 | Escherichia fergusonii | TGGAAGCGACCGGTATTCG | TGCTATGCAACCACGGCA | 59.86 | 59.97 | 156 | 81 |
100.00%
|
50.00%
|
| Primer 90 | Escherichia fergusonii | TGCTGAGCGAAACACTGGA | ACAAGGCCAACAGCAGCA | 59.55 | 60.12 | 232 | 81 |
100.00%
|
50.00%
|